It is undeniable truth that poor quality of work life is a big challenge in today’s higher learning education institutions which affects negatively the performance as well as attraction and retention of quality staff. This study focused on analyzing the level of quality of work life of academic staff of public universities and examining the factors affecting the attainment of Quality of work life in public universities in Dar es Salaam Tanzania. The study employed a case study design to provide thorough, in-depth, comprehensive, and well-ordered information concerning the social group understudy. A sample size of 138 respondents drawn from the population that was 210 academic managers and administrative staff members from Dar es Salaam University and Mzumbe University. Questionnaire and interviews were used to collect the required information. The findings revealed low quality of work life. The study recommended that universities should come up with mitigation measures to reduce the workload of academic staff since this was one of the leading factors affecting attainment of quality of work life.